| Vector |
Construct size (kb): 7.833000183105469 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514 |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: SV40 virus
Gene name: DNA Segment
Contains complete coding sequence?: Y
Strain: rhesus monkey
Tissue: brain, spleen, and lymph nodes
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 5.147
Cross references: DNA Seq. Acc.: AF038616
Nucleotides 1-5147 of the insert correspond to
nucleotides 1-5147 of AF038616. Insert lengths(kb): 5.146999835968018 Tissue: brain, spleen, and lymph nodes |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1, 2.7; BamHI--4.4, 3.4; SalI--7.8. Insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.44; BspHI--1.82, 2.68; EcoRI--1.69; HindIII--0.95, 1.4, 1.62, 3.38, 3.91, 5.08; NcoI--0.04, 0.24, 0.47; NdeI--3.71, 4.73; PstI--1.90, 3.11; PvuII--0.18, 1.62, 3.41. First protoarchetypal version of SV40 to be cloned and characterized. It has numerous nucleotide differences from SV40 reference strain 776. This is a viable clone (viral DNA, when excised from the plasmid using EcoRI, will form virus particles if the DNA is transfected into CV-1 cells or other permissive cells). Full-length genome of SV40 strain K661-1 (SV40-K661-1) was cloned into pUC-19 at the EcoRI site. |
