| 產(chǎn)品名稱 |
p300 |
| 商品貨號 |
B186539 |
| Designations |
p300 |
| Species |
Homo sapiens, human |
| Depositors |
W Stetler-Stevenson |
| Vector |
Construct size (kb): 3.263000011444092 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: BamHI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: MMP2
Other gene symbols: CLG4A; CLG4
Gene name: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase,
72kD type IV collagenase)
Contains complete coding sequence?: U
Chromosome: 16; Localization: 16 q12.2
Tissue: placenta
Type of DNA: cDNA
Insert 5' end: EcoRI
Insert 3' end: BamHI
Insert size (kb): 0.305
Cross references: Insert lengths(kb): 0.3050000071525574 Tissue: placenta Gene product: matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)(collagenase IVA (basement membrane), metalloproteinase stromelysin-2) [CLG4] |
| Insert Size (kb) |
0.305 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): KpnI--3.1, 0.2; BglI--1.85, 1.20, 0.25; XbaI--3.3; HindIII--3.3; BamHI/EcoRI--3.0, 0.3. The insert contains the following restriction sites (bp from the 5' end): BglI--24; HaeIII--27, 40, 51, 88; KpnI--168; MboI--134; NcoI--20; SacI--59. The insert corresponds to nt 1448-1747 of the published sequence. Corresponds to nt 1730-2030 of the near full length cDNA clone p16SPT19-1. Detects a transcript of 3.1 kb. |
| References |
Levy AT, et al. Increased expression of the Mr 72,000 type IV collagenase in human colonic adenocarcinoma. Cancer Res. 51: 439-444, 1991. PubMed: 1846313
Collier IE, et al. H-ras oncogene-transformed human bronchial epithelial cells (tbe-1)secrete a single metalloprotease capable of degrading basement membrane collagen. J. Biol. Chem. 263: 6579-6587, 1988. PubMed: 2834383
William Stetler-Stevenson, personal communication
|
