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Construct size (kb): 3.910000085830689

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC19
Intact vector size: 2.686
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Construction: pUC71K
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ', <-
MCS: EcoRI...HindIII, ->
promoter: lac, <-
replicon: pMB1, <-
marker(s): ampR, <-
Cross references: DNA Seq. Acc.: X02514

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: IGB
Genomic copy number: unique
Gene name: immunoglobulin-associated beta (B29)
Contains complete coding sequence?: Y
Chromosome: 17; Localization: 17 q23
Tissue: tonsil
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.224
Cross references: DNA Seq. Acc.: M80461

Insert lengths(kb): 1.223999977111816

Tissue: tonsil

Gene product: immunoglobulin-associated beta (B29) [IGB]

Restriction digests of the clone give the following sizes (kb): HindIII--3.9; EcoRI--2.7, 1.2; BglI--1.45, 1.35; PstI--3.9; KpnI--3.7, 0.2.

The insert contains the following restriction sites (nt from the 5' end): BglI--20, KpnI--117; NcoI--15, 1063; PvuII--81, 278, 464, 804.

Contains the complete coding sequence (nt 15-702).