ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Insert contains SacI (3), SphI (1), and HincII (1) sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.3, 1.1; HincII--4.2, plus smaller; HindIII--4.4; PstI--4.4; BamHI--4.4.
The protein resulting from in vitro translation of this cDNA (unglycosylated, with signal peptide) is precipitated by MAb 17D6 specific to Blast-1.
Detects human genomic restriction fragments of the following sizes (kb): EcoRI--9.3, 6.7, 3.4 (weak); HindIII--2.9, 2.1, 1.2, 0.8.
This is a full-length or close to full-length cDNA clone including 46 bp 5' untranslated and about 430 bp 3' untranslated sequence. The sequence predicts 5 N-glycosylation sites.
The insert is oriented such that transcription from the T7 promoter gives the sense strand.
References
Lai LW, et al. A gene correcting the defect in the CHO mutant Ade-H, deficient in a branch point enzyme (adenylosuccinate synthetase) of de novo purine biosynthesis, is located on the long arm of chromosome 1. Genomics 9: 322-328, 1991. PubMed: 2004783
Fisher RC, Thorley-Lawson DA. Characterization of the Epstein-Barr virus-inducible gene encoding the human leukocyte adhesion and activation antigen BLAST-1 (CD48). Mol. Cell. Biol. 11: 1614-1623, 1991. PubMed: 1847502
Staunton DE, Thorley-Lawson DA. Molecular cloning of the lymphocyte activation marker Blast-1. EMBO J. 6: 3695-3701, 1987. PubMed: 2828034
