| Vector |
Construct size (kb): 5.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKM3
Other gene symbols: ERK1
Gene name: protein kinase, mitogen activated 3 (MAP kinase 3; p44)
Contains complete coding sequence?: N
Chromosome: 16; Localization: 16
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert 5' end: HindIII
Insert 3' end: NotI
Insert size (kb): 1.8
Cross references: DNA Seq. Acc.: T09258; EST: EST07151; Primer: M13 Reverse;
THC id: THC97562
DNA Seq. Acc.: X60188; EST: EST07151
Nucleotides 1-348 of the insert correspond to
nucleotides 158-505 of X60188 with 99% identity. Insert lengths(kb): 1.799999952316284 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: mitogen activated protein kinase 3( p44), protein kinase, mitogen activated kinase 3, protein kinase, mitogen-activated 3 (MAP kinase 3) [ERK1] |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII+NotI--3.2, 0.94, 0.78; EcoRI--5.0. Based on the alignment with EMBL accession X60188 (1866 nt, CDS 73-1212), this clone appears to contain part of the coding region. The cDNAs (>95%) are cloned directionally with the 5' end adjacent to the M13 reverse primer and HindIII. |
| References |
Adams MD, et al. Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library. Nat. Genet. 4: 373-380, 1993. PubMed: 8401585
Mark D Adams, personal communication
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