| 產品名稱 |
Rotavirus A |
| 商品貨號 |
B185414 |
| Classification |
Reoviridae, Rotavirus, Rotavirus A |
| Deposited As |
Human rotavirus |
| Agent |
Rotavirus A |
| Strain |
HRV 248 |
| Applications |
Initially grow cells in MEM with 10% FCS, then use 0.1-1.0 MOI of virus to inoculate cells which have been rinsed with PBS to remove residual FCS. Incubate on a rocker plate. Primarily neutralized by hyperimmune guinea pig sera to serotypes 2 and 4, but is only recognized by monoclonal antibody to serotype 4. Enteric Research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
stool from a 17-month-old child, Bangladesh |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Comments |
Initially grow cells in MEM with 10% FCS, then use 0.1-1.0 MOI of virus to inoculate cells which have been rinsed with PBS to remove residual FCS. Incubate on a rocker plate. Primarily neutralized by hyperimmune guinea pig sera to serotypes 2 and 4, but is only recognized by monoclonal antibody to serotype 4. -70C or lower |
| Effect on Host |
Yes, in vitro effects: Cytopathic effects (cell rounding and sloughing) CPE: cell rounding and sloughing |
| Growth Medium |
Minimum essential medium (Eagle) in Earle's BSS (serum-free) with 1 to 5 micrograms/ml trypsin
|
| Recommended Host |
MA-104 cells (ATCC CRL-2378) Host of Choice:? MA-104? Host Range: MA-104 MA-104 cells (ATCC CRL-2378) |
| Growth Conditions |
Temperature: 37.0°C Duration: 1-5 days at 37C in MA-104 cells (ATCC CRL-2378), 5% CO2 |
| Cryopreservation |
Storage temperature: -70C or lower |
| Name of Depositor |
James N. Gamble Institute of Medical Research |
| U.S. Patent |
|
| U.S. Patent Number |
|
| Special Collection |
NCRR Contract |
| Source |
stool from a 17-month-old child, Bangladesh |
| References |
Ward RL. Human rotaviruses, vaccines and methods. US Patent 5,695,767 dated Dec 9 1997
|
