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Size (kb): 7.6250000000000000

Vector: pCM184 (plasmid)

Promoters: Promoter CMV

Marker(s):TRP1,ampR

Construct size (kb): 7.625

Features: marker(s): TRP1
marker(s): ampR
operator: tetO - seven repeats
other: tTA transcriptional activator
promoter: CMV
promoter: CYC1
replicon: ARS1
replicon: pMB1
MCS: BamHI...PstI
terminator: ADH1
terminator: CYC1
centromere: CEN4

YC-type (centromeric) shuttle vector

expression vector

Restriction digests of the clone give the following sizes (kb): BamHI--7.5; PstI--7.5; BamHI/XhoI--6.6, 0.76.

Yeast centromeric expression vector driven by the tetO-CYC1 promoter. One of 7 tetracycline-regulated expression vectors (ATCC 87656 - 87662) differing in the level of expression, mode of replication and selectable marker.

Temperature: 37.0°C

component of:ATCC 87663