| 產(chǎn)品名稱 | pHP1 plasmid in E. coli GC10 |
|---|---|
| 商品貨號(hào) | B185183 |
| Designations | pHP1 plasmid in E. coli GC10 |
| Species | Homo sapiens, human |
| Depositors | Roel Nusse |
| Applications | The product of this reaction was then PCR amplified again using another forward primer outside the protein encoding domain and a degenerate set of reverse primers to a highly conserved stretch FHWCC. |
| Vector | Construct size (kb): 3.95
Insert site: SmaI |
| Insert | DNA: cDNA
Gene: Wingless-type MMTV integration site family member 3, WNT3
Insert size (kb): 1.0 |
| Putative ID | wingless-related MMTV integration site 3 |
| Media | ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information | ?Frozen glycerol stock of Escherichia coli GC10 containing the plasmid. |
| Comments | This is a clone of the human WNT3 in the pBluescript M13 vector.
The insert was the result of a PCR amplification of mRNA from fetal tissue using a forward primer derived from the first exon of human WNT3 outside the coding domain and a set of degenerate reverse primers to the reverse complement of the mouse Wnt-3 gene corresponding to the last 4 amino acids and the stop codon. The product of this reaction was then PCR amplified again using another forward primer outside the protein encoding domain and a degenerate set of reverse primers to a highly conserved stretch FHWCC. Restriction digests of the clone gave the following results (in kb): NotI - 4; NotI/EcoRI - 3.0, 1.0; HindIII/XhoI - 4.0; SmaI - uncut; NotI/HindIII - 3.0, 1.0; HindIII - 4.0. - ATCC staff Due to the use of a gene internal 3' primer in the PCR reaction, the depositor did not retrieve the complete protein encoding sequence of human WNT3. Ref |
| References | Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993. |
| 序號(hào) | 文章標(biāo)題 | 操作 |
|---|---|---|
| 1 | ATCC細(xì)胞運(yùn)輸和處理 | 查看詳情 |
| 2 | 其他海外品牌代理 | 查看詳情 |
| 3 | ATCC代理 | 查看詳情 |
| 4 | 購(gòu)買須知 | 查看詳情 |
| 5 | 訂購(gòu)流程 | 查看詳情 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
Roelink H, et al. Molecular cloning and chromosomal localization to 17q21 of the human WNT3 gene. Genomics 17: 790-792, 1993.