| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb), ordered as in the genome: 5.764, 2.526, 1.951, 2.143, 2.042, 0.679, 0.621, 3.448, 1.163, 0.938, 0.570, 0.485, 0.947, 1.676, 0.885, 6.831, 2.565, 3.500, 1.567, 1.224. Confirmed to contain EcoRI/HindIII fragments of the following sizes (kb): 0.57, 0.63, 0.64, 0.77, 0.85, 0.92, 1.0, 1.2, 1.4, 1.6, 1.7, 2.0. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Overlaps clones with ATCC number(s): 70252, 70715, 70755. Corresponds to nt 245038-286800 of the sequence of chromosome VIII. |
| References |
Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229
Linda Riles, personal communication
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