Umbilical Cord-Derived Mesenchymal Stem Cells; Normal, Human
商品貨號
B184438
Organism
Homo sapiens, human
Tissue
Umbilical
Cell Type
Mesenchymal stem
Morphology
spindle-shaped, fibroblast-like
Growth Properties
adherent
Biosafety Level
1
[These primary cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment. Cells derived from primate lymphoid tissue may fall under the regulations of 29 CFR 1910.1030 Bloodborne Pathogens.
ATCC recommends that appropriate safety procedures be used when handling all primary cells and cell lines, especially those derived from human or other primate material. Detailed discussions of laboratory safety procedures are provided in Laboratory Safety: Principles and Practice, 2nd ed. (ASM Press, Washington, DC) (Fleming et al., 1995) and Caputo, J.L. Biosafety procedures in cell culture. (1988) J. Tissue Culture Methods 11:223.
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: Biosafety inMicrobiological and Biomedical Laboratories, 5th ed. HHS Publication No. (CDC) 93-8395. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online at http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.]
Human Material Precaution
All tissues used for isolation are obtained under informed consent and conform to HIPAA standards to protect the privacy of the donor’s personal health information. It is best to use caution when handling any human cells. We recommend that all human cells be accorded the same level of biosafety consideration as cells known to carry HIV. With infectious virus assays or viral antigen assays, even a negative test result may leave open the possible existence of a latent viral genome.
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
The cells are cryopreserved in the second passage to ensure the highest viability and plating efficiency.
Mesenchymal Stem Cell Basal Medium, when supplemented with Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low serum (ATCC PCS-500-040) components, provides an ideal cell system to propagate mesenchymal stem cells in low serum (2% FBS) conditions. When maintained under optimal growth conditions, ATCC Normal Human Umbical Cord-Derived Mesenchymal Stem Cells have been shown to be multipotent, capable of differentiating down the adipogenic, osteogenic and chondrogenic lineages.
Complete Growth Medium
Obtain one Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low serum from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium.
Obtain one bottle of Mesenchymal Stem Cell Basal Medium (485 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
Table 1. Mesenchymal Stem Cell Growth Kit for Adipose and Umbilical-derived MSCs - Low serum Components
