| 產品名稱 |
Fcwf-4 [Fcwf] |
| 商品貨號 |
B184268 |
| Organism |
Felis catus, cat |
| Tissue |
fetus, whole |
| Cell Type |
macrophage |
| Product Format |
frozen |
| Morphology |
spindle to stellate |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
peritonitis |
| Age |
fetus |
| Applications |
It is used to propagate tissue culture adapted Feline coronavirus (Feline infectious peritonitis virus, FIPV).
|
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This continuous feline cell line was developed in 1979. |
| Virus Susceptibility |
Feline infectious peritonitis virus
|
| Comments |
The cells possess some characteristics of macrophages (nonspecific esterase, phagocytic activity, Fc receptors). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 1 x 104 to 1 x 105 viable cells/cm2 is recommended. Do not exceed 1 x 106 cells/cm2.
- Incubate cultures at 37°C.
Interval: Maintain cultures at a cell concentration between 2 x 104 and 1 x 105 cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation |
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
|
| Population Doubling Time |
31 hrs |
| Name of Depositor |
NC Pedersen |
| Year of Origin |
1979 |
| References |
Jacobse-Geels HE, Horzinek MC. Expression of feline infectious peritonitis coronavirus antigens on the surface of feline macrophage-like cells. J. Gen. Virol. 64: 1859-2866, 1983. PubMed: 6886678
Pedersen NC, et al. Infection studies in kittens, using feline infectious peritonitis virus propagated in cell culture. Am. J. Vet. Res. 42: 363-367, 1981. PubMed: 6267959
|
