The insert contains the following restriction sites (in bp from the 5' end of the insert): BamHI--70 (5' untranslated region); NarI--234; SmaI--400; PvuII--597; PstI--737, 983. Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.25; BamHI--4.3; SmaI--3.8, 0.45; PvuII--3.6, 0.65; PstI--3.5, 0.75. The insert contains 99 nucleotides of 5' untranslated sequence and coding regions for a signal peptide of 18 amino acids and the first 379 amino acids of the mature protein. The insert detects a mRNA of 6.0 kb from rat smooth muscle cells. |
