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Quantitative Synthetic Eastern equine encephalitis virus RNA
Quantitative Synthetic Eastern equine encephalitis virus RNA
規格:
貨期:
編號:B183890
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Quantitative Synthetic Eastern equine encephalitis virus RNA
商品貨號 B183890
Agent Quantitative Synthetic Eastern equine encephalitis virus RNA
Applications ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine viral load.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Specification range: 1 x 105 to 1 x 106 copies/µL
100 μL per vial with Biomatrica RNAstable
Storage Conditions -70°C or colder
Intended Use The synthetically engineered sequence of the product constitutes intellectual property belonging to ATCC. Unauthorized use, including sequencing, modification, or reverse-engineering, of the product is expressly prohibited without prior ATCC consent.

Biomatrica Logo
Comments Manufactured under ISO 13485 guidance

Preparation includes fragments from capsid, envelope (E1 and E2), and nonstructural genes NSP1, NSP3, and 3’UTR.

The following primers and probe can be used with this nucleic acid preparation RefLambert AG, et al. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J. Clin. Microbiol. 41(1): 379-385, 2003. PubMed: 12517876:
Forward primer: ACACCGCACCCTGATTTTACA
Reverse primer: CTTCCAAGTGACCTGGTCGTC
Probe: FAM-TGCACCCGGACCATCCGACCT-BHQ
Special Collection RNA
References

Lambert AG, et al. Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays. J. Clin. Microbiol. 41(1): 379-385, 2003. PubMed: 12517876

Zink SD, et al. Quadraplex qRT-PCR assay for the simultaneous detection of Eastern equine encephalitis virus and West Nile virus. Diagn. Microbiol. Infect. Dis. 77(2): 123-132, 2013. PubMed: 23891222

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