| Vector |
Construct size (kb): 4.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK-
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CTH
Genomic copy number: unique
Gene name: cystathionase (cystathionine gamma-lyase)
Contains complete coding sequence?: U
Chromosome: 16; Localization: 16
Tissue: liver
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.1
Cross references: Insert lengths(kb): 1.100000023841858 Tissue: liver Gene product: cystathionine gamma-lyase(cystathionase (cystathionine gamma-lyase)) [CTH] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.1; BglII--4.1; BamHI--4.1; HindIII--4.1; XbaI--4.1. The insert contains the following restriction sites (bp from the 5' end): BglI--88; BamHI--114; AvaII--128; AvaI--198; ScaI--278; DraI--333; XmnI--589; BstEII--840; BspHI--959; NsiI--968; BglII--1065; XbaI--1068; HindIII--1076. Contains a complete coding sequence, as a variant missing 132 bp included in other cDNAs derived from liver. Detects a 2.5 kb mRNA in human liver. Transcription from the T3 promoter gives the sense strand. Enzymes not detecting polymorphism: TaqI, XbaI, BglI, RsaI, HindIII, MspI, PstI, EcoRV, ScaI, SacI, BamHI, NcoI, Sau3A, PvuII. |
| References |
Chua AO, et al. Expression cloning of a human IL-12 receptor component--a new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. 153: 128-136, 1994. PubMed: 7911493
Lu Y, et al. Cloning and nucleotide sequence of human liver cDNA encoding for cystathionine gamma-lyase. Biochem. Biophys. Res. Commun. 189: 749-758, 1992. PubMed: 1339280
The last 13 nucleotides of the coding sequence are missing.---ATCC staff
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