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pv-sis [SISR12]
pv-sis [SISR12]
規格:
貨期:
編號:B182634
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pv-sis [SISR12]
商品貨號 B182634
Designations pv-sis [SISR12]
GenBank Number

J02396

Species simian sarcoma virus (type C)
Depositors HM Temin, SA Aaronson
Vector
Construct size (kb): 5.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 1.200000047683716
Gene product: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)(platelet-derived growth factor, beta polypeptide) [SIS]
Alleles: B1, B2, B1, B2
Insert Size (kb) 1.200
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; PstI--4.8, 1.2.
A 1.0 kb PstI/XbaI fragment contains v-sis but no helper virus sequences.
Contains nt 4052-5122 of the published sequence, including most of coding sequence for v-sis, a polyadenylation signal, the v-sis helper virus junction, the coding sequence for p15E, and part of an LTR.
References

Fabisiak JP, et al. Increased expression of PDGF-B (c-sis) mRNA in rat lung precedes DNA synthesis and tissue repair during chronic hypoxia. Am. J. Respir. Cell Mol. Biol. 1: 181-189, 1989. PubMed: 2696512

Radice P, et al. Loss of heterozygosity in human germinal tumors. Cytogenet. Cell Genet. 52: 72-76, 1989. PubMed: 2612217

Kochevar DT, et al. Low level amplification of c-sis and c-myc in a spontaneous osteosarcoma model. Cancer Lett. 53: 213-222, 1990. PubMed: 2208081

Robbins KC, et al. Molecular cloning of integrated simian sarcoma virus: genome organization of infectious DNA clones. Proc. Natl. Acad. Sci. USA 78: 2918-2922, 1981. PubMed: 6265924

Devare SG, et al. Nucleotide sequence of the simian sarcoma virus genome: demonstration that its acquired cellular sequences encode the transforming gene product p28sis. Proc. Natl. Acad. Sci. USA 80: 731-735, 1983. PubMed: 6298772

梅經理 17280875617 1438578920
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周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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