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pART1/N795 [pART1-N795]
pART1/N795 [pART1-N795]
規格:
貨期:
編號:B181876
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pART1/N795 [pART1-N795]
商品貨號 B181876
Designations pART1/N795 [pART1-N795]
GenBank Number

M14053

Species Rattus norvegicus, rat
Depositors KR Yamamoto
Applications
in another host, produces protein glucocorticoid receptor
Vector
Construct size (kb): 10.10000038146973
Insert
DNA: cDNA
Insert lengths(kb): 2.799999952316284
Tissue: hepatoma cell line J2.17 (HTC derivative)
Gene product: glucocorticoid receptor DNA binding factor 1 [GRL]
Target Gene: glucocorticoid receptor
Insert Size (kb) 2.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.3, 2.8; EcoRI--4.4, 3.9, 1.2, 0.5; HindIII--4.6, 2.2, 1.7, 0.9, 0.3.
The insert contains the following restriction sites (approximate nt from the 5' end): BglI--0.4, 0.6; HindIII--1.0, 1.36, 2.24, 2.4; PstI--1.6, 1.64; EcoRI--2.3.
Extends from 24 nt upstream of the major initiation codon to 360 nt downstream of the termination codon and thus encodes a protein of 795 amino acids.
pART1 was constructed by inserting a 1.2 kb Schizosaccharomyces pombe ars fragment into the EcoRI site of pUC118 and a 2.2 kb fragment containing Saccharomyces cerevisiae LEU2 into the HindIII site.
A 700 bp SphI/PstI fragment containing the adh promoter of Sc. pombe was inserted into polylinker sites. Transcription is driven from the SphI site toward the remaining PstI, SalI and BamHI sites through the insert sequence.
References

Picard D, et al. An inducible expression vector for both fission and budding yeast. Gene 86: 257-261, 1990. PubMed: 2108906

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
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