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phi2-22
phi2-22
規格:
貨期:
編號:B181009
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 phi2-22
商品貨號 B181009
Designations phi2-22
Species Homo sapiens, human
Depositors M Litt
Vector
Construct size (kb): 44.5
Insert
DNA: genomic
Insert lengths(kb): 15.0
Gene product: DNA Segment, single copy [D11S35]
Alleles: A2, B1, A1, B2
Insert Size (kb) 15.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried bacteria-free lysate
Comments
A SalI digest gave the following sizes (kb): 20, 15.5, 9.
Insert contains repeated sequences and must be prehybridized with total human DNA before it will detect single-copy bands. Standard hybridization and washing conditions work well, with a final wash in 0.1X SSC/0.1% SDS at 65C.
The A locus is also detected with TaqI, BglII, PstI.
References

Hoefsloot LH, et al. HindIII/EcoRI polymorphism in the GAA gene. Nucleic Acids Res. 18: 5921, 1990. PubMed: 1977144

McClure CM, et al. RFLPs revealed by probes from genomic libraries specific for chromosome regions 11q and 16q. Am. J. Hum. Genet. 39: A1629, 1986.

Maslen CL, et al. Seven polymorphic loci mapping to human chromosomal region 11q22-qter. Genomics 2: 66-75, 1988. PubMed: 2898428

Michael Litt, personal communication

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