Cyanidioschyzon merolae P. De Luca, R. Taddei & L. Varano
商品貨號
B180748
Deposited As
Cyanidioschyzon merolae
Strain Designations
10D
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Hot spring, Sardinia, Italy
Product Format
frozen
Storage Conditions
Frozen Cultures: -70°C for 1 week; liquid N2 vapor for long term storage
Freeze-dried Cultures: 2 to 8°C
Live Cultures: See Protocols section for handling information
Genome Sequenced Strain
Yes
Comments
Genome sequencing strain
Medium
ATCC® Medium 2861: Acidic Allen Medium
Growth Conditions
Temperature: 42°C
Cryopreservation
Harvest and Preservation
Harvest cells from a culture which is at or near peak density by centrifugation at ~800 x g for 10 min.
Adjust concentration of cells to 2 x 107 - 2 x 108 mL in fresh growth medium. If the concentration is too low, centrifuge at ~800 x g for 5 minutes and resuspend the cell pellet with a volume of supernatant to yield the desired cell concentration.
While cells are centrifuging, prepare a 10% (v/v) solution of sterile methanol in fresh growth medium.
Mix the cell suspension and the methanol solution in equal portions. The final concentration will be 107 – 108 cells/mL and 5% (v/v) of methanol. The time from the mixing of the cell preparation and methanol stock solution before the freezing process is begun should be no less than 5 min and no more than 15 min.
Dispense in 0.5 mL aliquots into 1.0 - 2.0 mL sterile plastic screw-capped cryovials.
Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
Store in either the vapor or liquid phase of a nitrogen refrigerator.
To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after it is thawed.
Remove the vial from the water bath immediately after thawing. Aseptically transfer the contents of the ampule into 5 mL of fresh ATCC medium 2861.
Incubate the tube on a 15° horizontal slant with the cap screwed on loosely (loosened one half turn) at 42°C under constant light.
Maintain as described above.
Name of Depositor
T. Kuroiwa
Chain of Custody
ATCC <-- T. Kuroiwa
References
Ohta N, et al. Structure and organization of the mitochondrial genome of the unicellular red alga Cyanidioschyzon merolae deduced from the complete nucleotide sequence. Nucleic Acids Res. 26(22): 5190-5198, 1998. PubMed: 9801318
Ohta N, et al. Complete sequence and analysis of the plastid genome of the unicellular red alga Cyanidioschyzon merolae. DNA Res. 10(2): 67-77, 2003. PubMed: 12755171
Matsuzaki M, et al. Genome sequence of the ultrasmall unicellular red alga Cyanidioschyzon merolae 10D. Nature 428(6983): 653-657, 2004. PubMed: 15071595
Nozaki H, et al. A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae. BMC Biol. 5: 28, 2007. PubMed: 17623057
