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Construct size (kb): 12.0

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAcMP1
Intact vector size: 10.052
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: BamHI
Polylinker sites:
Host range: insect cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: basic protein
replicon: pMB1
Cross references:

DNA: cDNA

DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PRKCB2
Other gene symbols: PKCB; PRKCB
Gene name: protein kinase C, beta 2
Contains complete coding sequence?: Y
Tissue: brain, temporal cortex
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 2.0
Cross references:

Insert lengths(kb): 2.0

Tissue: brain, temporal cortex

Gene product: protein kinase C, beta 2(protein kinase C, beta II) [PKCB]

Restriction digests of the clone give the following sizes (kb): BamHI--10.0, 2.0; EcoRI--6.6, 3.0, 2.6; HindIII--10.0, 1.1, 1.0; PstI--11.0, 1.3; PvuII--3.8 (doublet), 2.4, 1.5, 0.6.

The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--1.96; EcoRV--1.78; HindIII--1.86; NcoI--1.23; NotI--0.48; PstI--0.15, 1.34; PvuII--0.69; SacI--1.10.

Contains the complete coding sequence only (nt 65-2086 of GenBank M13975).