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Size (kb): 3.4000000953674320

Vector: pJS133 (plasmid)

Promoters: Promoter lac

Construction: CAT (from pKK232-8), pUC18

Marker(s):ampR,cmlR

Construct size (kb): 3.400000095367432

Features: insert detection: lacZ'
marker(s): ampR, cmlR
promoter: lac
replicon: pMB1

contains easily purifiable cassette(s) for construction

There is an EcoRI site within the CAT gene.

Restriction digests of the clone give the following sizes (kb): XbaI--3.5; HindIII--2.75, 0.75.

Plasmid with a promoterless CAT (chloramphenicol acetyltransferase) cassette that can be excised for use in genetic constructions. CAT expression reflects transcriptional activity only; translation stops in all 3 reading frames.

Temperature: 37.0°C