| Vector |
Construct size (kb): 2.95799994468689 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: D0S13E
Genomic copy number: unique
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: U
Sequence homology: Homo sapiens orthodenticle
Tissue: fetal brain (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb):
Cross references: DNA Seq. Acc.: M85320; EST: EST01828; Primer: M13 Forward Tissue: fetal brain (from Bento Soares) Gene product: orthodenticle [Otd] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.45, 1.2; HindIII--5.0, 1.65; PstI--6.4, 0.25; BamHI--6.65. EST01828 includes sequences that translate into homeodomains, with the most similarity to Drosophila otd. It also includes a splice acceptor sequence that begins where the sequence of EST1828 diverges from otd. EST01828 matches PIR accession A35912 with 52.77% identity and 75% similarity over the sequence range: 242-135. Isolated from a directional library constructed by B. Soares, Columbia University. First strand synthesis was primed with an oligo(dT)-NotI primer. Much of the priming occurred by the NotI portion of the primer, so although the clones are directional, the NotI site is not necessarily the 3' end of the message. This sequence record is for Drosophila melanogaster orthodenticle (Otd). |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
Burglin TR, Barnes TM. Introns in sequence tags. Nature 357: 367-368, 1992. PubMed: 1350660
J C Venter, personal communication
|
