| 產品名稱 |
pAS1-SNF1 |
| 商品貨號 |
B179673 |
| Designations |
pAS1-SNF1 |
| Species |
Homo sapiens, human |
| Depositors |
SJ Elledge |
| Applications |
in another host, produces protein SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Vector |
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pAS1
Intact vector size: 7.100
Type of vector: phagemid
Vector end: XhoI
Vector end: XhoI
Cloning sites: NdeI NcoI SfiI SmaI BamHI SalI
Polylinker sites: NdeI NcoI SfiI SmaI BamHI SalI
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: ADC1 (ADH), ->
other: GAL4 DNA-binding domain, ->
epitope tag: hemagglutinin (HA), ->
restriction site: EcoRI, ->, 4315
MCS: NdeI...SalI, ->, 4395-4422
terminator: ADC1
replicon: pMB1
marker(s): ampR
replicon: 2 micron
marker(s): TRP1, ->
replicon: f1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: SNF1
Gene name: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end: XhoI
Insert end: XhoI
Insert size (kb):
Cross references: Gene product: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase [SNF1] Target Gene: SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Related Products |
component of:ATCC 87014 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): XhoI--8.5, 1.0; EcoRI--9.5; BamHI--7.2, 2.35; HindIII--4.8, 3.0, 0.9, 0.7; BamHI/HindIII--4.8, 2.2, 0.9, 0.7, 0.5. Used as a negative control in the 2-hybrid cloning system. |
