Restriction digests of the clone give the following sizes (kb): EcoRI--4.8; HindIII--4.8; BamHI--4.8; PstI--4.4, 0.4. This probe hybridizes to human but not rodent cardiac actin sequences under the following conditions: 4X SSC; 50 mM NaH2P04; 5 X Denhardts, 10% dextran sulfate at 65C. Wash at 0.5X SSC, 0.1% SDS, 65 C. The insert corresponds to intervening sequence 4 of the gene. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
