| Vector |
Construct size (kb): 6.199999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: APXL
Gene name: apical protein, Xenopus laevis-like
Contains complete coding sequence?: N
Tissue: retina, adult
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 3.223
Cross references: DNA Seq. Acc.: X83543
Nucleotides 1-3223 of the insert correspond to
nucleotides 403-3625 of X83543. Insert lengths(kb): 3.223000049591065 Tissue: retina, adult Gene product: apical protein, Xenopus laevis-like [APXL] Target Gene: apical protein, Xenopus laevis-like |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 3.0; BamHI--4.7, 1.5; HindIII--3.7, 2.5. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.0; BglI--2.3, 2.45; HindIII--2.65; SacI--0.95, 2.5, 3.0; SalI--0.05; PstI--1.05, 1.75, 1.8; PvuI--0.95; PvuII--1.0, 1.25, 2.1; XhoI--1.35, 2.9, 2.95. Contains a portion of the coding sequence, overlapping AR16 (ATCC 99508) at the 5' end and AR24 (ATCC 99512) at the 3' end. The coding region of GenBank X83543 corresponds to nt 91-4941. |
