| 產品名稱 |
Bovine herpesvirus 1 |
| 商品貨號 |
B178873 |
| Classification |
Herpesviridae, Varicellovirus |
| Deposited As |
Infectious bovine rhinotracheitis |
| Agent |
Bovine herpesvirus 1 |
| Strain |
Los Angeles |
| Common Name |
Infectious bovine rhinotracheitis virus, Bovine rhinotracheitis virus |
| Applications |
Produces hemolysin but not HA. Stable at -60°C. Single antigenic group. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Images |
 |
| Comments |
Produces hemolysin but not hemagluttinin. |
| Effect on Host |
CPE, rounding and sloughing |
| Recommended Host |
EBTr (NBL-4) (ATCC® CCL-44™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3 days |
| Name of Depositor |
DG McKercher, UC Davis School of Veterinary Medicine |
| Source |
Dairy cattle, Los Angeles County, California, 1956 |
| References |
Madin SH, et al. Isolation of the infectious bovine rhinotracheitis virus. Science 124: 721-722, 1956. PubMed: 13371311
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