| 產(chǎn)品名稱 |
hMT-IIA [MT2A, p84H] |
| 商品貨號(hào) |
B178794 |
| Designations |
hMT-IIA [MT2A, p84H] |
| GenBank Number |
J00271
|
| Species |
Homo sapiens, human |
| Depositors |
M Karin |
| Vector |
Construct size (kb): 7.599999904632568 |
| Insert |
DNA: genomic Insert lengths(kb): 3.200000047683716 Gene product: metallothionein 2(metallothionein II) [MT2] Alleles: A1, A2, B1, B2, A1, A2, B1, B2 |
| Insert Size (kb) |
3.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--4.6, 2.25, 0.3; SalI--7.6; PstI--6.6, 0.8; HindIII--4.3, 3.1. Insert includes entire coding sequence, 2 introns, 5' and 3' flanking regions. It spans about 900 bp. Expression is inducible by Cd++, Zn++, and glucocorticoids. Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with. |
| References |
Richards RI, et al. Structural and functional analysis of the human metallothionein-IA gene: Differential induction by metal ions and glucocorticoids. Cell 37: 263-272, 1984. PubMed: 6327055
Karin M, Richards RI. Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene. Nature 299: 797-802, 1982. PubMed: 7133118
Hyland VJ, et al. A 5' flanking region of the metallothionein, MT2A, gene identifies two moderately frequent RFLPs. Nucleic Acids Res. 15: 1350, 1987. PubMed: 2881275
Barbara Weiffenbach, personal communication
|
