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produces protein RNA polymerase

Construct size (kb): 7.099999904632568

DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBR322
Intact vector size: 4.363
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: EcoRI ClaI HindIII EcoRV BamHI SphI SalI XmaIII NruI BspMI
BsmI StyI AvaI BalI BspMII PvuII Tth111I NdeI AflIII PpaI
PstI PvuI ScaI SspI AatII
Polylinker sites:
Construction: pBR313
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): tetR
replicon: pMB1
marker(s): ampR
Cross references: DNA Seq. Acc.: J01749

DNA: genomic

DESCRIPTION OF INSERT COMPONENT:
Genome: bacteriophage T7
Gene symbol: gene 1
Genomic copy number: unique
Gene name: RNA polymerase
Contains complete coding sequence?: U
Type of DNA: genomic
Insert end: Modification: BamHI linkers
Insert end: Modification: BamHI linkers
Insert size (kb): 2.7
Cross references: DNA Seq. Acc.: J02518

Insert lengths(kb): 2.700000047683716

Gene product: RNA polymerase [gene 1]

Target Gene: RNA polymerase

Restriction digests of the clone give the following sizes (kb): BamHI--4.4, 2.8; BglII--7.2; EcoRI--7.2; PstI--7.2; HindIII--7.2.

The insert extends from nucleotides 3145 to 5841 of T7 DNA.

Gene 1 was sequenced from T7 wild-type DNA. The junctions between gene 1 and pBR322 in pAR1173 were sequenced.

This construct expresses a low level of T7 RNA polymerase, enough to complement an amber mutant.

pAR1173 was made by inserting a BglII linker into the BamHI site 5' of gene 1 in pAR1151. A BamHI site between the BglII site and gene 1 was lost, resulting in a BamHI-BglII-gene 1-BamHI construction. Sequences, such as promoters,

inserted in the BglII site will remain with gene 1 as a single fragment upon BamHI digestion.