| 產品名稱 |
p3-21 [pTD3-21] |
| 商品貨號 |
B178583 |
| Designations |
p3-21 [pTD3-21] |
| Species |
Homo sapiens, human |
| Depositors |
M Lalande |
| Applications |
To resolve the largest two alleles of the TaqI RFLP, the depositor uses 0.6% agarose gels, 0.8 V/cm, run at 4C for 5 days. |
| Vector |
Construct size (kb): 6.599999904632568 |
| Insert |
DNA: genomic Insert lengths(kb): 2.200000047683716 Gene product: DNA Segment, single copy [D15S10] Alleles: A1, A2, A3 |
| Insert Size (kb) |
2.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Insert contains the following sites separated by (kb): HindIII- 0.62- BamHI- 0.12- EcoRI- 1.46- HindIII. Restriction digests of the clone give the following sizes (kb): HindIII--4.4, 2.2; BamHI--4.7, 1.9; EcoRI--5.8, 0.8; SalI--6.6; EcoRI/SalI--3.6, 2.2, 0.8. To resolve the largest two alleles of the TaqI RFLP, the depositor uses 0.6% agarose gels, 0.8 V/cm, run at 4C for 5 days. |
| References |
Donlon TA, et al. Isolation of molecular probes associated with the chromosome 15 instability in the Prader-Willi syndrome. Proc. Natl. Acad. Sci. USA 83: 4408-4412, 1986. PubMed: 3012567
Nicholls RD, et al. Restriction fragment length polymorphisms with proximal 15q and their use in molecular cytogenetics and the Prader-Willi syndrome. Am. J. Med. Genet. 33: 66-77, 1989. PubMed: 2568752
Nicholls RD, Lalande M. Three allele TaqI RFLP for probe 3-21 [D15S10] on chromosome 15q. Nucleic Acids Res. 17: 10140, 1989. PubMed: 2574845
Marc Lalande, personal communication
|
