| Applications |
Animals were immunized against exotoxin A of Pseudomonas aeruginosa by infection with strain PA220. The antibody binds specifically for the exotoxin A of Pseudomonas aeruginosa. It displays specificity for exotoxin A from strain PA103 and strain PAO1 and PAO-PR1, a derivative of PAO1. The antibody shows high specificity and reactivity for native and denatured exotoxin A in ELISA assays. Both TC1 (ATCC CRL-2493) and TC31 (ATCC CRL-2494) antibodies neutralize exotoxin A toxicity against CHO cells in an in vitro assay but only TC31 protected mice infected with strain PA220 in an in vivo model. A hybridoma producing an anti idiotypic antibody against TC31 is available (ATCC CRL-2485). |
| Comments |
Animals were immunized against exotoxin A of Pseudomonas aeruginosa by infection with strain PA220. Spleen cells were fused with Sp2/0-Ag14 cells. The antibody binds specifically for the exotoxin A of Pseudomonas aeruginosa. It displays specificity for exotoxin A from strain PA103 and strain PAO1 and PAO-PR1, a derivative of PAO1. The antibody shows high specificity and reactivity for native and denatured exotoxin A in ELISA assays. Both TC1 (ATCC CRL-2493) and TC31 (ATCC CRL-2494) antibodies neutralize exotoxin A toxicity against CHO cells in an in vitro assay but only TC31 protected mice infected with strain PA220 in an in vivo model. A hybridoma producing an anti idiotypic antibody against TC31 is available (ATCC CRL-2485). |
| References |
Galloway DR, et al. Production and characterization of monoclonal antibodies to exotoxin A from Pseudomonas aeruginosa. Infect. Immun. 44: 262-267, 1984. PubMed: 6425217
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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