Primary Subcutaneous Pre-adipocytes; Normal, Human
商品貨號
B178456
Organism
Homo sapiens, human
Tissue
Mature adipose tissue
Morphology
Spindle-shaped, fibroblast-like
Growth Properties
Adherent
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
ATCC® Human Pre-Adipocytes are cryopreserved at the second passage to ensure the highest viability and plating efficiency. ATCC® Primary Cell Solutions™ cells, media, supplements and reagents are quality tested together to guarantee optimum performance and reliability.
Complete Growth Medium
Obtain one Fibroblast Growth Kit-Low Serum from the freezer; make sure that the caps of all components are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium.
Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the indicated volume of each growth kit component, as indicated in Table 1, to the bottle of basal medium using a separate sterile pipette for each transfer.
