| 產品名稱 |
pMW1 plasmid in E. coli |
| 商品貨號 |
B178381 |
| Designations |
pMW1 plasmid in E. coli |
| Depositors |
Amgen, Inc., F Lin, Amgen, Inc. |
| U.S. Patent |
|
| Biosafety Level |
1 |
| Host |
Distribution host: Escherichia coli JM103 |
| Vector Information |
Size (kb): 12.00 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: unknown
Intact vector size:
Type of vector: plasmid
Cloning sites:
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): kanR, tetR
Cross references: Vector: pMW1 (plasmid) Marker(s):kanR,tetR Construct size (kb): 12.0 Features: marker(s): kanR, tetR
replicon: pSH1
repressor gene: cI857 |
| Applications |
contains sequence repressor protein cI857 mutation |
| Comments |
Temperature sensitive, grow at 28C. A plasmid to provide a temperature sensitive lambda cI857 repressor for regulated expression of cloned sequences from a lambda promoter. Has a pSHI replicon, compatible with colE1 vectors. |
| Media |
ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
|
| Growth Conditions |
Temperature: 28.0°C |
| References |
Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987
|
| Shipped |
freeze-dried |
