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pGIBS-PHE
pGIBS-PHE
規(guī)格:
貨期:
編號(hào):B178335
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGIBS-PHE
商品貨號(hào) B178335
Designations pGIBS-PHE
GenBank Number

M24537

Species Bacillus subtilis (Ehrenberg) Cohn
Depositors M Follettie
Applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector Construct information:
Total size of construct (kb): 4.2
Marker: ampR
Promoter giving the sense strand: T3
Promoter giving the antisense strand: T7
Excise insert: XhoI + NotI

Vector information:
Vector: pBluescript II KS+
Vector type: phagemid
Vector size (kb): 2.961
Markers: ampR
Vector end (5’): XhoI
Vector end (3’): NotI
Insert Insert size (kb): 1.32
Gene: pheB
Source: Bacillus subtilis
DNA: genomic
Genbank accession: M24537 (Nucleotides 1-1318 of the insert correspond to nucleotides 2017-3334 of M24537)
Insert end (5’): XhoI linker
Insert end (3’): BamHI/poly(A)/NotI linker
Insert Size (kb) 1.320
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Frozen glycerol stock of ?E. coli containing the phagemid
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 2017 - 3334 of Genbank M24537.
The insert contains the following restriction sites (approximate kb from the 5' end): XmnI--0.36; AvaI--0.89; EcoRI--1.05.
Restriction digests of the clone give the following sizes (kb): NotI--4.4; NotI/XhoI--3.0, 1.4; XhoI--4.4.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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