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pHLC3.11 [pHLC3/11]
pHLC3.11 [pHLC3/11]
規格:
貨期:
編號:B178292
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 pHLC3.11 [pHLC3/11]
商品貨號 B178292
Designations pHLC3.11 [pHLC3/11]
Species Homo sapiens, human
Depositors GH Fey
Vector
Construct size (kb): 7.199999809265137
Insert
DNA: cDNA
Insert lengths(kb): 4.300000190734863
Gene product: complement component 3 [C3]
Alleles: D1, D2
Insert Size (kb) 4.300
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: freeze-dried
Comments
A ClaI/SalI double digest yields fragments of 2.9 kb (vector), 2.4 kb and 1.8 kb (insert).
Insert can be removed by SalI/ClaI double digest, cutting flanking vector sequences. Insert contains a SalI site.
Insert contains 4342 bp representing the coding sequence for approx. 90% of C3 measured from the COOH end. Includes COOH region of beta chain and all of coding sequences for alpha chain.
References

Botto M, et al. EcoRI polymorphism in the human third complement component (C3) gene. Nucleic Acids Res. 18: 2833, 1990. PubMed: 1971096

de Bruijn MH, Fey GH. Human complement component C3: cDNA coding sequence and derived primary structure. Proc. Natl. Acad. Sci. USA 82: 708-712, 1985. PubMed: 2579379

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