The insert includes the following sites (with nucleotide positions according to the published sequence): BglII--193, 286; NcoI--325; PstI--397; SpeI--539. Restriction digests of the clone give the following sizes (kb): XbaI--3.7; BglI--3.7; PstI--3.0, 0.35 (doublet); SacI--3.7; EcoRI/BamHI--3.0, 0.75; XhoI--3.0, 0.75; KpnI--3.0, 0.75. This insert was subcloned from pXM-hJE34, so the KpnI insert includes XhoI, SacI, and PstI sites at the 5' end and XhoI and PstI sites at the 3' end from pXM. The insert includes 24 nucleotides of poly(A) tail. The insert is oriented such that transcription from the SP6 promoter gives the sense strand. |
