| 產品名稱 |
Human coxsackievirus A3 |
| 商品貨號 |
B178244 |
| Classification |
Picornaviridae, Enterovirus, Enterovirus A |
| Agent |
Human coxsackievirus A3 |
| Strain |
Olson |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Images |
 |
| Comments |
Derived by adaptation from human amnion cell product ATCC® VR-163™ to RD cells (ATCC® CCL-136™). ATCC® VR-163™ and ATCC® VR-1799™ have not been compared for sequence or infectivity. |
| Effect on Host |
CPE, rounding and sloughing |
| Recommended Host |
RD (ATCC CCL-136) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-85% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 10-15 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days |
| Name of Depositor |
HA Wenner, University of Kansas Medical Center; ATCC |
| Source |
Stool of 2 year old with nuchal rigidity and hamstring muscle spasm, New York, 1948 |
| References |
Sickles GM, Dalldorf G. Serologic differences amoung strains of the coxsackie group of viruses. Proc. Soc. Exp. Biol. Med. 72(1): 30, 1949. PubMed: 15391667
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