| 產品名稱 | UACC-3199 |
|---|---|
| 商品貨號 | B178036 |
| Organism | Homo sapiens, human |
| Tissue | mammary gland/breast duct; derived from metastatic site: axillary lymph node |
| Product Format | frozen |
| Morphology | epithelial-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | infiltrating ductal carcinoma of the breast |
| Age | 58 years old |
| Gender | female |
| Ethnicity | Caucasian |
| Applications | This cell line is an excellent tool for research into aberrant cytosine methylation of CpG island sites and BRCA1 repression in sporadic breast cancer. It is also very useful as a methylated BRCA1 control. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | UACC-3199 was derived from a 58 year-old female with infiltrating ductal carcinoma of the breast metastatic to the left axillary lymph nodes. |
| Clinical Data | female
The patient had a family history of breast cancer.
58 years old
Caucasian |
| Receptor Expression | epidermal growth factor receptor (EGF), expressed estrogen receptor, not expressed progesterone receptor, not expressed |
| Oncogene | c-erbB-2 positive |
| Genes Expressed | c-erbB-2 positive |
| Tumorigenic | YES |
| Comments | The patient had a family history of breast cancer. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium (ATCC® 30-2008). To make the complete growth medium, add the following components to the base medium:
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium renewal: Every 3 to 4 days
Growth Conditions: Cells grow very slowly. Cultures reach confluence in 2 to 3 weeks. Avoid inoculating vessels below recommended seeding density. |
| Cryopreservation | Freeze medium: complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C
Atmosphere: air, 100% |
| STR Profile | CSF1PO: 12 D13S317: 11 D16S539: 12 D5S818: 13 D7S820: 10 THO1: 9.3 TPOX: 10, 13 vWA: 15, 17 Amelogenin: X |
| Name of Depositor | K Brown |
| Year of Origin | November 1994 |
| References | Rice JC, et al. Transcriptional repression of BRCA1 by aberrant cytosine methylation, histone hypoacetylation and chromatin condensation of the BRCA1 promoter. Nucleic Acids Res. 28(17): 3233-3239, 2000. PubMed: 10954590 Rice JC, et al. Aberrant methylation of the BRCA1 CpG island promoter is associated with decreased BRCA1 mRNA in sporadic breast cancer cells. Oncogene 17(14): 1807-1812, 1998. PubMed: 9778046 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
