| Vector |
Construct size (kb): 3.599999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: tyrosine kinase receptor substrate p120 isolog
Contains complete coding sequence?: U
Sequence homology: Homo sapiens phosphoprotein p120
Tissue: fetal brain, female, 17-18 wk (Stratagene 936206)
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 0.56
Cross references: DNA Seq. Acc.: T05030; EST: EST02918; Primer: M13-21; THC
id: THC92337
DNA Seq. Acc.: Z17804
Nucleotides 1-315 of the insert correspond to
nucleotides 1272-1586 of Z17804 with 86% identity.
PIR: S28498
Nucleotides 2-337 of the insert correspond to
nucleotides 311-422 of S28498 with 87% identity. Insert lengths(kb): 0.5600000023841858 Tissue: fetal brain, female, 17-18 wk (Stratagene 936206) Gene product: phosphoprotein p120 [P120*] |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII--3.6; EcoRI--3.0, 0.56. Based on the alignment with EMBL accession Z17804 (4636 nt, CDS 343-3070), this clone appears to contain part of the coding region. |
