| 產品名稱 |
HHCPQ46 |
| 商品貨號 |
B177732 |
| Designations |
HHCPQ46 |
| GenBank Number |
M78205
|
| Species |
Homo sapiens, human |
| Applications |
Based on restriction analysis and the alignment with GenBank accession Y00052 (723 nt, CDS 15-512), it appears that the fragment adjacent to the T7 promoter contains the complete cDNA, but that the clone is chimeric. |
| Vector |
Construct size (kb): 5.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: PPI*
Other gene symbols: PPI*
Gene name: peptidyl-prolyl cis-trans isomerase (cyclophilin, FK506
binding protein)
Contains complete coding sequence?: U
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 2.8
Cross references: DNA Seq. Acc.: M78205; EST: EST01798; Primer: M13 Forward;
THC id: THC125696
DNA Seq. Acc.: Y00052; EST: EST01798
Nucleotides 1-383 of the insert correspond to
nucleotides 3-385 of Y00052 with 99% identity. Insert lengths(kb): 2.799999952316284 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: peptidyl-prolyl cis-trans isomerase (cyclophilin, FK506 binding protein) [PPI*] |
| Insert Size (kb) |
2.800 |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.95, 1.95, 0.8. The insert contains multiple EcoRI fragments. It is important to note these may have been derived from different cDNAs. Based on restriction analysis and the alignment with GenBank accession Y00052 (723 nt, CDS 15-512), it appears that the fragment adjacent to the T7 promoter contains the complete cDNA, but that the clone is chimeric. |
| References |
Adams MD, et al. Sequence identification of 2,375 human brain genes. Nature 355: 632-634, 1992. PubMed: 1538749
J C Venter, personal communication
|
