| 產品名稱 |
pRBBP1-III cDNA |
| 商品貨號 |
B177674 |
| Designations |
pRBBP1-III cDNA |
| GenBank Number |
S57162
|
| Species |
Homo sapiens, human |
| Depositors |
GA Otterson |
| Applications |
contains sequence retinoblastoma-binding protein 1, isoform III |
| Vector |
Construct size (kb): 6.699999809265137 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: BamHI
Vector end: HindIII
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RBBP1
Gene name: retinoblastoma-binding protein 1, isoform III
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.8
Cross references: DNA Seq. Acc.: S57162 Insert lengths(kb): 3.799999952316284 Gene product: retinoblastoma-binding protein 1, isoform III [RBBP1] Target Gene: retinoblastoma-binding protein 1, isoform III |
| Insert Size (kb) |
3.800 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.7; BamHI/HindIII--3.8, 3.0; EcoRI-- 3.0, 2.6, 1.2; HindIII--6.7; PstI--4.4, 1.8, 0.46. |
| References |
Otterson GA, et al. Alternative splicing of the RBP1 gene clusters in an internal exon that encodes protential phosphorylation sites. Oncogene 8: 949-957, 1993. PubMed: 8455946
Fattaey AR, et al. Characterization of the retinoblastoma binding proteins RBP1 and RBP2. Oncogene 8: 3149-3156, 1993. PubMed: 8414517
|
