| 產品名稱 |
Canine distemper virus |
| 商品貨號 |
B177146 |
| Classification |
Paramyxoviridae, Morbillivirus |
| Agent |
Canine distemper virus |
| Strain |
Snyder Hill (cell culture adapted) |
| Applications |
It is used primarily as a challenge strain to test dogs for immunity to the disease.
ATCC VR-526, deposited by L. E. Carmichael, was originally isolated from the brain of a dog with clinical distemper in September, 1950.
This virus produces neurological signs with regularity only when inoculated intracerebrally.
It does not produce hemagglutinin or hemolysin.
Respiratory research |
| Positive for Mycoplasma |
No |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Neurological signs are produced with regularity only when inoculated intracerebrally. This strain does not produce hemagglutinin or hemolysin. It is used primarily as a challenge strain to test dogs for immunity to this disease. Cross-immunity tests in dogs have shown it to be similar to other classical strains of CDV. |
| Effect on Host |
CPE, vacuolization and rounding of cells, sloughing |
| Recommended Host |
Vero cells (ATCC® CCL-81™)
|
| Growth Conditions |
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 70% - 90% confluent (not 100% confluent).
Incubation: 5 to 7 days at 37°C, a 5% CO2 in air atmosphere is recommended |
| Name of Depositor |
LE Carmichael, ATCC |
| Special Collection |
ATCC |
| Source |
ATCC® VR-1587™ was derived at ATCC from ATCC® VR-526™ by adaptation to growth in Vero cells (ATCC® CCL-81™). ATCC® VR-526™, deposited by L.E. Carmichael, was isolated from brain of dog with clinical distemper, September, 1950. Because it was propagated in animal tissue ATCC will no longer offer that product. |
| References |
Gillespie JH. . Am. J. Vet. Res. 17: 103, 1956. PubMed: 13259024
|
