| 產品名稱 |
Adeno-associated virus 1 |
| 商品貨號 |
B176973 |
| Classification |
Parvoviridae, Dependovirus, Adeno-associated virus-1 |
| Deposited As |
Adeno-associated (satellite) virus type 1 |
| Agent |
Adeno-associated virus 1 |
| Strain |
none |
| Applications |
Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
Pool of RhMk TC infected with SV-15 virus |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder
(or colder) |
| Comments |
Depends upon adenovirus for replication. Suggest VR-848, Adenovirus Type 7A, as helper virus, which produces CPE on ATCC CRL-1573 cells. *Helper virus may be inactivated by heating to 56C for 30 min. |
| Effect on Host |
in vitro effects: Adeno-associated virus shows no independent cytopathic effects Adenovirus CPE, refractile rounding and sloughing; AAV shows no independent CPE. Yes, in vitro effects: Adenovirus cytopathic effects, refractile rounding and sloughing |
| Growth Medium |
Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 98%; fetal bovine serum, 2%
|
| Recommended Host |
Recommended Host:? HEK-293 (ATCC? CRL-1573TM) |
| Growth Conditions |
Atmosphere: 5% CO2 in air recommended Temperature: 37.0°C Duration: 2-4 days; For best results cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent). |
| Cryopreservation |
Storage temperature: -70°C |
| Name of Depositor |
WP Parks |
| Special Collection |
ATCC |
| Source |
Pool of RhMk TC infected with SV-15 virus |
| References |
Atchison RW, et al. Adenovirus-associated defective virus particles. Science 149: 754-756, 1965. PubMed: 14325163
or colder
or colder
|
