| Insert |
DNA: cDNA Insert lengths(kb): 3.200000047683716 Tissue: umbilical vein endothelium cell line Gene product: von Willebrand factor pseudogene [VWF] Alleles: K2, M1, A4, C1, C2, D1, D2, G1, G2, K1, K2, M1, M1, M2, A1, A2, A3, A5, A6, A7, A4, C1, C2, D1, D2, G1, G2, K1, M1, M2, A1, A2, A3, A5, A6, A7, M2, M2, , , , A1, A2, A1, A1, A2, A2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.7; BamHI--3.9, 1.6; PstI--5.1, 0.56; HindIII--5.9; SacI--4.4, 1.2. Probes I (ATCC 59782/59783), II (ATCC 59784/59785), III (ATCC 59786/59787) and IV (ATCC 59788/59789) constitute non-overlapping fragments of the human von Willebrand factor cDNA sequence that together span essentially the entire 9 kb cDNA. The 1.2 kb BamHI/FspI fragment constitutes 'probe II' and corresponds to amino acids 144-544 of von Willebrand factor. Insert is a 3.2 kb fragment from lambda HvWF4. The 3' 1.9 kb of the insert corresponds to vWF sequences. The 5' 1.3 kb is an uncharacterized cloning artifact. |
| References |
Shelton-Inloes BB, et al. Evolution of human von Willebrand Factor: cDNA sequence polymorphisms, repeated domains, and relationship to von Willebrand Antigen II. Biochem. Biophys. Res. Commun. 144: 657-665, 1987. PubMed: 3495266
Shelton-Inloes BB, et al. Gene deletions correlate with the development of alloantibodies in von Willebrand disease. J. Clin. Invest. 79: 1459-1465, 1987. PubMed: 3033024
J E Sadler, personal communication
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