| Vector |
Construct size (kb): 4.099999904632568 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript SK-
Intact vector size: 2.960
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: KpnI ApaI DraII XhoI (SalI HincII AccI) ClaI HindIII EcoRV
EcoRI PstI SmaI BamHI SpeI XbaI EagI NotI SacII BstXI SacI
Polylinker sites: SacI BstXI SacII NotI EagI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII (ClaI Bsp106I) (HincII AccI SalI) XhoI
(Eco0109I DraII ApaI) KpnI
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, ->
insert detection: lacZ', <-
primer site: M13-20, ->
promoter: T7, ->
MCS: KpnI...SacI, ->
promoter: T3, <-
promoter: lac, <-
primer site: M13 reverse, <-
replicon: pMB1
marker(s): ampR, <-
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: NEFH
Genomic copy number: unique
Gene name: neurofilament, heavy polypeptide (200kD)
Contains complete coding sequence?: N
Chromosome: 22; Localization: 22 q12.1-q13.1
Tissue: hippocampus, female, 2 years, oligo-dT and random primed
(Stratagene 936205), subtracted
Type of DNA: cDNA
Insert end: Modification: EcoRI linkers
Insert end: Modification: EcoRI linkers
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: M78157; EST: EST01749; Primer: M13 Forward;
THC id: THC76212
DNA Seq. Acc.: X15309; EST: EST01749
Nucleotides 1-357 of the insert correspond to
nucleotides 2425-2069 of X15309 with 99% identity. Insert lengths(kb): 1.200000047683716 Tissue: hippocampus, female, 2 years, oligo-dT and random primed (Stratagene 936205), subtracted Gene product: neurofilament, heavy polypeptide (200kD)(neurofilament, heavy polypeptide) [NEFH] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.2; BamHI--4.1; PstI--4.1. Based on the size of the insert and the alignment with GenBank X15309 (2542 nt, exon 4), this clone appears to contain part of exon 4. |
