| 產品名稱 |
pSP64-VIII |
| 商品貨號 |
B176444 |
| Designations |
pSP64-VIII |
| Species |
Homo sapiens, human |
| Depositors |
Genetics Institute, Inc., JJ Toole, Genetics Institute, Inc. |
| Vector |
Construct size (kb): 10.19999980926514 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSP64
Intact vector size: 2.999
Type of vector: plasmid
Vector end: SalI
Vector end: SalI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
promoter for in vitro transcription: SP6, ->
MCS: HindIII...EcoRI, ->
enhancer: none
replicon: pMB1
marker(s): ampR
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: human
Gene symbol: F8C
Genomic copy number: unique
Gene name: coagulation factor VIIIc, procoagulant component
(hemophilia A)
Contains complete coding sequence?: U
Chromosome: X; Localization: X q28
Tissue: fetal liver
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 7.2
Cross references: Insert lengths(kb): 7.199999809265137 Tissue: fetal liver Gene product: coagulation factor VIIIc, procoagulant component (hemophilia A) [F8C] Alleles: F3, E1, E1, E2, F1, F2, A1, A2 |
| Insert Size (kb) |
7.200 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
PvuI and SmaI are unique restriction sites. Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 3.0, 2.6; EcoRI--4.9, 3.4, 1.8, 0.4. The insert contains cDNA sequences from just before the initiator ATG (nucleotide 150-152) to nucleotide 7422 (200 bp downstream from the terminator at nucleotide 7201). The insert detects a single mRNA of about 9 kb from human liver. This was constructed by ligating several independently isolated cDNA clones and adding a SalI linker at nucleotide 145. |
| References |
Toole JJ Jr.. Novel procoagulant proteins. US Patent 4,868,112 dated Sep 19 1989
Toole JJ, et al. Molecular cloning of a cDNA encoding human antihaemophilic factor. Nature 312: 342-347, 1984. PubMed: 6438528
John J Toole, personal communication
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
