Restriction digests of the clone give the following sizes (kb): BamHI--21.0, 13.5 (doublet); EcoRI--21.0, 13.5 (doublet); SalI--20.3, 12.0, 9.4, 6.0. Vector permitting positive selection (Spi) of recombinants. Useful for conversion to phagemid (without selection). Permits construction using partial filling-in. The restriction sites to be used for conversion to phagemid are: SalI SacII BglI SfiI NotI XmaIII. Cloning capacity: 18.9 kb. The sizes of the major components are (kb): arms--20.3, 9.2; stuffer fragment--13.7; phagemid segments--0.7 (longer arm), 3.9. The order of the major features in this plasmid is: arm - SalI/XmaIII - XbaI/MCS/NaeI - stuffer - NaeI/MCS/SacI - M13 ori - ampR - pMB1 ori - XmaIII/SalI - arm. |
