| 產品名稱 | MDCK.1 |
|---|---|
| 商品貨號 | B176147 |
| Organism | Canis familiaris, dog |
| Tissue | kidney; distal tubule |
| Product Format | frozen |
| Morphology | fibroblast-like |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age | adult |
| Applications | This cell line is insensitive to epsilon toxin from C.perfringens and is a control for MDCK.2 (ATCC CRL-2936) |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | hyperdiploid canine cell line with a modal chromosome number of 76 with low polyploidy rate. Several unidentifiable marker chromosomes were present in most of the cells examined. |
| Images |  |
| Derivation | Cell line was derived by cloning (limited dilution) the parental cell line MDCK (ATCC CCL-34). |
| Antigen Expression | E-cadherin (epithelial cell adhesion molecule), expressed Zona Occludens (ZO-1) (tight junction protein), not expressed CD29, expressed CD18, not expressed fibroblast-specific protein (FSP), not expressed cytokeratin (CK1, 4, 5, 6, 8, 10, 13, 18, 19),expressed |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation ratio: A subcultivation ratio of 1: 3 to 1:8 is recommended. Medium renewal: Every 2 to 3 days.
|
| Cryopreservation | Freeze medium: Complete growth medium, 95%; DMSO, 5% liquid nitrogen vapor phase |
| Population Doubling Time | approximately 14 hours |
| Name of Depositor | Y. Reid, E. Cedrone and E-Eckard-Amar, ATCC |
| Year of Origin | Jan 2007 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
