| 產(chǎn)品名稱 |
pRBBP1-I |
| 商品貨號 |
B176110 |
| Designations |
pRBBP1-I |
| GenBank Number |
S57153
|
| Species |
Homo sapiens, human |
| Depositors |
GA Otterson |
| Applications |
contains sequence retinoblastoma-binding protein 1, isoform I |
| Vector |
Construct size (kb): 3.900000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEM-4
Intact vector size: 2.870
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Polylinker sites: HindIII SphI PstI SalI AccI HincII XbaI BamHI AvaI SmaI KpnI
SacI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
MCS: EcoRI...HindIII, ->
promoter for in vitro transcription: T7, <-
replicon: pMB1
marker(s): ampR, <-
promoter for in vitro transcription: SP6, ->
Cross references: DNA Seq. Acc.: X65303 |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RBBP1
Gene name: retinoblastoma-binding protein 1, isoform I
Contains complete coding sequence?: N
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.04
Cross references: DNA Seq. Acc.: S57153
Nucleotides 1-1040 of the insert correspond to
nucleotides ~1560-2600 of S57153. Insert lengths(kb): 1.039999961853027 Gene product: retinoblastoma-binding protein 1, isoform I [RBBP1] Target Gene: retinoblastoma-binding protein 1, isoform I |
| Insert Size (kb) |
1.040 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 1.0; EcoRI--4.0; HindIII--4.0; PstI--3.0, 0.84; SacI--4.0. |
| References |
Otterson GA, et al. Alternative splicing of the RBP1 gene clusters in an internal exon that encodes protential phosphorylation sites. Oncogene 8: 949-957, 1993. PubMed: 8455946
|
