| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.7; BglI--2.0, 1.2, 0.35; PvuII--2.35, 0.6, 0.3, 0.2; PstI--2.7, 0.7; HindIII--3.4. The insert contains the following restriction sites (nt from the 5' end): BglI--83; PvuII--431, 612; PstI--588. Contains the complete coding sequence, from nt 25 to nt 447. The mature peptide is encoded from nt 85 to nt 444. |