Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
Comments
This strain is indistinguishable antigenically from strain MM and Mengo encephalomyocarditis virus.
Effect on Host
CPE, rounding and sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 15-20 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 2-4 days
Name of Depositor
W Brandt
Source
Chimpanzee, Florida, 1944
Year of Origin
1944
References
Helwig FC, Schmidt EC. A filter-passing agent producing interstitial myocarditis in anthropoid apes and small animals. Science 102: 31-33, 1945.