Restriction digests of the clone give the following sizes (kb): EcoRI--4.5, 2.0; BamHI--5.5, 0.65, 0.55; HindIII--6.6. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.00,0.48; BglII--0.03; HindIII--1.42; KpnI--1.51. The processed polypeptide shows over 90% sequence identity to mouse egasyn (Eg, Es22). |
